Senta Reichelt

Affinity Chromatography: Methods and Protocols - Methods in Molecular Biology Senta Reichelt 3rd ed. 2015 edition

Marc Notes: Expanded and updated from the first and second editions, this third edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. Table of Contents:1. Affinity Chromatography: A Historical David S. Hage and Ryan Matsuda Part I: General Modes and Ligands of Affinity Chromatography2. Protein Purification by Aminosquarylium Cyanine-Affinity ChromatographyVania C. Graca, Fani Sousa, Paulo F. Santos and Paulo S. Almeida3. One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography Cunxi Wang, Thomas C. Lee, Kathleen S. Crowley, and Erin Bell4. Antibody Purification from Human Plasma by Metal-Chelated Affinity MembranesHandan Yavuz, Nilay Bereli, Fatma Y lmaz, Canan Armutcu and Adil Denizli5. Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic LigandCatarina Caramelo-Nunes and Candida T. Tomaz6. Cell Affinity Separations on Microfluidic DevicesYan Gao, Wenjie Li, Ye Zhang and Dimitri Pappas7. Aptamer-modified: Magnetic Beads in Affinity Separation of Proteins Guohong Zhu and Johanna-Gabriela Walter8. The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of ProteinsThomas Schmidt and Arne Skerra9. Robotic High-throughput Purification of Affinity-tagged Recombinant ProteinsSimone C. Wiesler and Robert O. J. Weinzierl Part II: Matrices in Affinity Chromatography10. Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of GlycoproteinsBen Mann11. Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural ProductsYaqi Chen, Zhui Chen and Yi Wang12. Mixed-bed Affinity Chromatography: Principles And MethodsEgisto Boschetti and Pier Giorgio Righetti13. Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing BiomoleculesQianjin Li and Zhen Liu Part III: Macroporous Polymers as Matrix in Affinity Chromatography14. Introduction to Macroporous Cryogels Senta Reichelt15. Cryogels with Affinity Ligands as Tools in Protein PurificationSolmaz Hajizadeh and Bo Mattiasson16. Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity ChromatographyNaveen Kumar Singh, Roy N. DSouza, Noor Shad Bibi and Marcelo Fernandez-Lahore17. Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease AdsorptionMurat UYGUN18. A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human SerumGozde Baydemir and Mehmet Odaba 19. Molecularly Imprinted Cryogels for Human Serum Albumin DepletionMuge Andac, Igor Yu Galaev, Handan Yavuz and Adil Denizli20. Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption PropertiesEcaterina Stela Dragan and Maria Valentina Dinu Part IV: Spectroscopy, Binding Studies and Molecular Modelling21. Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum AlbuminRyan Matsuda, Jeanethe Anguizola, Krina S. Hoy and David S. Hage22. Accurate protein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume SamplesChristian Kohler, Raphael Recht, Marc Quinternet, Frederic de Lamotte, Marc-Andre Delsuc, and Bruno Kieffer23. Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis Chenchen Lu and Zhen Liu24. Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity ChromatographyJiwen Zhang, Haiyan Li, Lixin Sun, and Caifen Wang25. Molecular Modeling of the Affinity Chromatography of Monoclonal AntibodiesMatteo Paloni and Carlo Cavallotti"Jacket Description/Back: The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. "Affinity Chromatography: Methods and Protocols, Third Edition" guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, " Affinity Chromatography: Methods and Protocols, Third Edition" is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity."